Study of Fibrinogen Levels in Blood after Infused a Modified Plasmin in Rabbit

 Study of Fibrinogen Levels in Blood after Infused a Modified Plasmin in Rabbit

Plasmin is a two chains molecule connected by two Disulfide bridge, Plasmin results of plasminogen (MW 92 Kdal) activation by rupture of the union Arg560-Val-561. Plasminogen activation is produced by activators of plasminogen as t-PA (tissue - Plasminogen Activator) or Streptoquinase. Plasmin has interactions with substances, proteases and inhibitors as α2-antiplasmin with to a lesser activity α2-macroglobulin for its regulation in blood. Methods: ELISA Detection of Plasmin the test principle applied in this kit is Sandwich enzyme immunoassay. New Zealand white rabbits weighing 3 Kg were separated in two groups 10 (placebo) and 10 (Plasmin study). The determination of fibrinogen with thrombin clotting time is based on the Clauss method. Bleeding the ear artery after 30, 60 and 240 min of the infusion in both groups. Fibrinogen levels in plasma were analyzed in both groups. Native polyacrylamide gel electrophoresis was performed to run and compare the same protein in different temperature and pH precipitation conditions. SDS-PAGE was performed by the method of Laemmli using a 10% polyacrylamide gel.