Myo-Inositol Oxygenase Expression Associate with Oxidative Stress and Renal Function Decline in Patients with Diabetic Nephropathy

Background: Diabetic kidney disease (DKD) is the leading cause of renal failure. The diagnostic preferred, urinary albumin is nonspecific and insensitive. There is urgently want for finding a renal-precise biomarker that could be detectable early sufficient and a capability target for therapeutic intervention.

Method: The expression of Myo-Inositol Oxygenase(MIOX)in kidney tissues from DKD and minimum-change nephropathy patients were detected via immunohistochemistry and the connection between MIOX expression and medical parameters of DKD have been analyzed by Pearson correlation evaluation. Then, the extent of MIOX in both of serum and urine had been tested via ELISA, and the correlation of MIOX in serum and urine and scientific parameters have been also analyzed.

Results: Immunohistochemistry showed that MIOX was localized to the proximal renal tubule and MIOX expression is significantly up regulated in kidney of patients with DKD ,compared with controls(p<0.01). Moreover, linear regression analysis revealed MIOX expression in the kidney of DKD patients was positively related to tubulointerstitial lesion score, ROS generation, urea nitrogen(BUN), creatinine, blood glucose, glycosylated hemoglobin and 24-hour urine protein. In addition, ELISA analysis showed that the levels of MIOX in serum and urine of DKD patients were significantly increased .The correlation analysis indicated that the expression of MIOX in both serum and urine were also positively related to creatinine, blood glucose, glycosylated hemoglobin, and 24-hour urine protein.

Conclusion: These dates indicate that MIOX is associated with ROS accumulation and kidney injury and plays an important role in the development of DKD. Furthermore, serum or urine MIOX maybe a new diagnostic biomarker and therapeutic target.