Comparison of Real-Time RTPCR Assay with Direct Sequencing for Detection of Sensitivity or Resistant to Oseltamivir in Influenza A/H3N2 Viruses

Comparison of Real-Time RTPCR Assay with Direct Sequencing for Detection of Sensitivity or Resistant to Oseltamivir in Influenza A/H3N2 Viruses

Background: Currently, there is an increasing risk of a worldwide influenza A virus epidemics not only influencing livestock but also humans, then research about the prophylaxis and treatment of these viruses and detection of drug resistant mutants is a must. Nowadays Oseltamivir “the neuraminidase (NA) inhibitor” is the suitable drug for preventing and treatment of these virus infections. Drug resistant viruses has already detected by mutations of the enzyme's active site in the neuraminidase gene that cause amino acid substitutions predominantly at positions like 119, 274, 292. The objective of this research was the comparison of the Real-time RT-PCR assay with direct sequencing for detection of sensitivity or resistant to Oseltamivir in Influenza A/H3N2 viruses.