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Hindi Research Article, J Biocatal Biotransformation Vol: 2 Issue: 1
Purification, Immobilization, Stabilization and Characterization of Commercial Extract with β-galactosidase Activity
| Chiara Calandri1, Daniela P. Marques2, Cesar Mateo4, Alfonso V. Carrascosa3, Jose M. Guisán4, Gloria F. Lorente3* and Benevides C. Pessela3 | |
| 1Scuela Normale Superiore di Pisa. Piazza dei Cavalieri 7, 56126, Pisa, Italy | |
| 2Department of Foods and Nutrition, Faculty of Pharmacy, Universidade Estadual Paulista Julio de Mesquita Filho – UNESP, Highway Araraquara Jau- Km 1, 14801 902, Araraquara, São Paulo, Brazil | |
| 3Department of Biotechnology y Food Microbiology, Instituto de Investigaciones en Ciencias de los Alimentos, CIAL, C / Nicolás Cabrera 9, 28049, Cantoblanco, Campus UAM, Madrid, Spain | |
| 4Department Biocatálisis, Institute of catalysis-CSIC, Campus UAM. Cantoblanco, 28049 Madrid, Spain | |
| Corresponding author : Dr. Gloria F. Lorente Department of Biotechnology y Food Microbiology, Instituto de Investigaciones en Ciencias de los Alimentos, Madrid, Spain Tel: +341 562 2900; Fax: + 341 564 4853 E-mail: gfl@ifi.csic.es |
|
| Received: January 10, 2013 Accepted: February 14, 2013 Published: February 21, 2013 | |
| Citation: Calandri C, Marques DP, Mateo C, Carrascosa AV, Guisán JM, et al. (2013) Purification, Immobilization, Stabilization and Characterization of Commercial Extract with ß-galactosidase Activity. J Biocatal Biotransformation 2:1. doi:10.4172/2324-9099.1000104 |
Abstract
Tyrosinase and Tyrosinase Inhibitors
From a commercial enzyme extract juice with purpose to stabilize it in the industry, has been able to extract an extract rich in beta-galactosidase activity. It has been designed to extract and purification protocol based on polyethyleneimine SUPPORTS agarose activated with next purification yields 80%.