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Hindi Research Article, J Vet Sci Med Diagn Vol: 4 Issue: 1
Protection against P. multocida B:2 using Recombinant 87kDa OMP Periplasmic Protein
| M Bagath1,2*, Megha Kadam Bedekar1, Pallab Chaudhuri1, Varalakshmi S3 and PP Goswami1 |
| 1Genetic engineering of Bacteria Lab, Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izat Nagar, Bareilly, Uttar Pradesh-243122, India |
| 2Animal Physiology Division, National Institute of Animal Nutrition and Physiology, Adugodi, Bangalore-560030, India |
| 3Dairy Microbiology, National Dairy Research Institute, Adugodi, Bangalore-560030, India |
| Corresponding author : Dr. Bagath M Scientist, Animal Nutrition Division, National Institute of Animal Nutrition and Physiology, Adugodi, Bangalore-560030, India Tel: +91-9035865536; Fax: +91-080-25711420 E-mail: bbagath@gmail.com |
| Received: June 28, 2014 Accepted: September 01, 2014 Published: September 03, 2014 |
| Citation: Bagath M, Megha KB, Pallab C, Varalakshmi S, Goswami P.P. (2015) Protection against P. multocida B:2 using Recombinant 87kDa OMP Periplasmic Protein. J Vet Sci Med Diagn 4:1. doi:10.4172/2325-9590.1000151 |
Abstract
Protection against P. multocida B: 2 using Recombinant 87kDa OMP Periplasmic Protein
To clone and express Pasteurella. multocida (P. multocida) encoding 87 kDa omp gene in prokaryotic (secretory) vector and to study the immunogenicity of expressed protein.The genomic DNA was isolated from P. multocida B:2. Specific primers were designed to suite secretory prokaryotic vector system. PCR amplified gene was cloned in pGEMT-EASY vector (Promega, USA) and subcloned into pBAD/gIIIA prokaryotic expression vector. The protein was purified and Passive mouse protection test challenge was conducted in mice.
