Nucleic acid purification from plant tissues for viral detection

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Eric Ting, Samantha Lewis, Laurence Delauriere, Becky Carroll, Elisa Ruiz, Ellyn Lepinski, Giuseppe Durante, Caroline Chatillon, Doug Wieczorek,
Celine Menager, Eric B Vincent and Trista Schagat

Promega Corporation, Singapore
Promega Europe Training and Applications Laboratory, France
International Plant Analysis and Diagnostics srl., Loc. Cascina Codazza, Italy
Qualiplante, France
Promega Corporation, USA

: J Plant Physiol Pathol

Abstract

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The diverse range of plant species and tissue types found in nature present a unique set of obstacles for nucleic acid isolation from botanical samples. In addition, the carry-over of substances present in certain plants can interfere with quantitation and downstream amplifications. These difficulties highlight the need for robust, reliable chemistries for molecular biology studies with plants. We present several solutions to purify and amplify nucleic acid from a variety of plant types and tissues with a focus on agriculturally relevant applications.

Biography

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Eric Ting present work in promega corporation, Singapore

Email: eric.ting@promega.com