In vitro effects of coffee polyphenol on intracellular oxidant/antioxidant balance of hepatocytes

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Merzouk Saidi Amel, Merzouk Hafida, Medjdoub Amel, Loukidi Bouchra and Merzouk Sid Ahmed

Abou-Bekr Belkaïd University, Algeria

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Abstract

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Introduction: The aim of this study was to evaluate the cytoprotective effect of chlorogenic acid (CGA) the most important coffee polyphenol against rat hepatocytes oxidative injury induced by H2O2 free radical generator in vitro. Material & Methods: Hepatocytes were isolated from liver obtained from Wistar rats and were cultured in RPMI medium at 37°C in a 5% CO2 humidified atmosphere for 48 h, in the presence or the absence of chlorogenic acid at two concentrations (10 and 50 μM). Proliferation index, LDH release, oxidative balance (catalase, reduced glutathione, malondialdehyde, and carbonyl proteins), and the presence of micronucleus were investigated with biochemical methods. Results: The results showed that free radical generators induced a significant decrease in hepatocyte proliferation and in glutathion contents and catalase activity, a significant increase in LDH release with changes in malondialdehyde, carbonyl proteins and micronucleus contents. CGA supplementation enhanced hepatocyte proliferation and reduced the degree of cell membrane injury and intracellular oxidative stress. Hepatocytes pre-treated with CGA were more resistant to the free radical damages. The higher dose of CGA was more potent for reducing intracellular oxidative stress and also for preventing it. Conclusion: In conclusion, our observations provide evidence that CGA had beneficial effects on liver function by correcting and also by preventing oxidative stress. 

Biography

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