Gamma radiation synthesis of silver nanoparticles stabilized in chitosan for agricultural application

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Le Quang Luan and Duong Hoa Xo

Biotechnology Center, Vietnam

: J Nanomater Mol Nanotechnol

Abstract

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Gamma rays irradiation is a convenient method for preparation of colloidal silver nanoparticles (AgNPs). The analytical results from UV-vis spectra and TEM images showed that the AgNPs with particle sizes of approx. 10 nm was successfully prepared by irradiating the solution of 10 mM AgNO3 in 1% chitosan at 28 kGy. The in vitro antifungal activity of AgNPs against three kinds of phytopathogenesis funguses was investigated. The results showed that the addition of 20 - 100 ppm AgNPs inhibited the growth of Phytophthora capsici (a fungus causing the serious Foot Rot disease on pepper plant) on CRA medium 22.6 - 92.9%, respectively. For Corynespora cassiicola fungus causing Corynespora leaf fall disease on rubber plant, its growth was inhibited from 6.3 to 100%, corresponded to the supplementation of AgNPs with concentrations from 10 to 90 ppm. In addition, the test on Fusarium oxysporum causing Fusarium wilt disease on tomato also pointed out that the treatment of AgNPs with concentration from 20 to 30 ppm also inhibited the growth of this pathogenesis fungus from 50.5 to 94.9%, respectively. Thus, the AgNPs product prepared by gamma radiation method using chitosan as a stabilizer may potentially be used as an antimicrobial agent in agriculture for controlling the pathogens on plants due to several reasons such as the environmentally-friendly production technology, highly antimicrobial effect and safety for using.

Biography

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Email: lequangluan@gmail.com