Fabrication and characterization for promoting osteoblast differentiation using bioactive substance from fish frame

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Seong-Yeong Heo and Won-Kyo Jung

Pukyong National University, Republic of Korea

: J Pharm Drug Deliv Res

Abstract

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In order to utilize fish byproducts in a calcium and phosphate supplement, Johnius belengerii frame composed of flesh and skeleton discarded from industrial processing were degraded by pepsin in acetic acid solution. In current study, we report the osteogenic effect of fish frame extracts (FBE) from Johnius belengerii in MC3T3-E1 pre-osteoblast. We designed composite scaffolds consisting of poly-caprolactone (PCL) and FBE fabricated by three axis plotting system for bone regeneration, The effect of FBE coated scaffolds (1 and 3%) on various mechanical properties and charactertistics including the morphology image, FT-IR analysis, tensile properties and drug releases were investigated. Moreover, the in vitro biocompatibilities of the FBE coated scaffolds were examined using MC3T3-E1 pre-osteoblast. On the scaffold surface, cell attachment, proliferation, mineralization and osteogenic factors were determined. At the results, the PCL/FBE combined scaffolds showed cell attachment, proliferation, mineralization and osteogenic factors than the PCL scaffold. Consequently, the FBE combined scaffold suggents further investigation a potential biomedical engineering field due to enhancement of osteogenesis.

Biography

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Seong-Yeong Heo has completed his MSc from Pukyong National University in Korea. He has studied Marine Life Science and Tissue Engineering. Currently, he is doing research on tissue regenerative scaffold fabricated by three axis plotting system and cell signaling investigated by western blot and RT-PCR analysis. Additionally, he has published 3 papers in reputed journals.

Email: hsyadsl@naver.com