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Hindi Evaluation of anti-oxidant activity of Malus domestica fruit extract from Kashan area
Sara Jelodarian, Abdolrasoul Ebrahimabadi, Ahmad Khalighi and Hossain Batooli
Bharati Vidyapeeth Deemed University, India
: J Plant Physiol Pathol
Abstract
Ceropegia maccanni Ansari is a rare and endangered herbaceous plant belonging to the family Ascepiadaceae. The study was conducted with an objective to establish a protocol for in vitro propagation of plantlets from node, internode and leaf explants of Ceropegia. Multiple shoots were obtained by studying the influence of cytokinins BA (6-benzyladenine) and Kin (Kinetin) and auxins IAA (Indole-3-acetic acid), NAA (2-Naphthalene acetic acid) and 2,4-D (2,4-Dicholorophenoxyacetic acid) alone and in combinations. The best response for multiple shoot induction was obtained in nodal explants on Murashige and Skoogs medium supplemented with 7.5 uM BA and Kin (3.2±0.3 and 3.1±0.3 shoots per explants respectively). The shoots were rooted on half strength MS (Murashige and Skoog’s) medium fortified with either IAA or NAA. However optimum percent frequency of shoots producing roots and number of roots per shoots was observed on liquid half strength MS medium fortified with 5% sucrose and 2.0 uM IAA in combination. Healthy regenerated were selected for hardening. Theses plantlets were transferred directly to the field with 100 percent success rate
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