Endophytic fungi Alternaria species-1 p-coumaric acid induces anticancer activity on HeLa cancer cell line by initiating mitochondrial-dependent apoptosis pathway

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Vikil Ramesh

Dayananda Sagar College of Engineering, India

: J Nanomater Mol Nanotechnol

Abstract

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In the present study, endophytic fungi, Alternaria alternata water extract was used to analyze phytochemical by qualitative and GC-MS methods. Synthesized the silver nanoparticles were characterized by UV-Vis spectra, XRD, FTIR, DLS, EDX and SEM to know biophysical properties and evaluated for antioxidant, anticancer and nano-toxicological studies. The endophytic fungal water extract yielded biologically important phytochemicals in qualitative method and the GC-MS yielded five different types of phytochemicals. The color was changed to dark brown when silver nitrate incubated with Alternaria alternata water extract. Typical silver nanoparticles formations were confirmed using UV-visible spectra at 420nm and were spherical shaped ranging size 30-40nm. The AaAgNPs showed significant antioxidant activity was compared with ascorbic acid and butylated hydroxytoluene in all the four methods (DPPH, scavenging activity of H2O2 and nitric oxide and reducing power). The AaAgNPs significantly reduced the viability of the HeLa cancer cell line and enhanced the caspase-3, 7, 8 and 9 activities at the highest level, assumed to be AaAgNPs induces mitochondrial mediated apoptosis. But, AaAgNPs had not inhibited the growth of green gram and also exhibited anti-hemolysis and heme-biocompatible activity on human blood RBCs. Together of all results, the study provided use of endophytic fungi Alternaria alternata in the synthesis of nanoparticles and their significant antioxidant and nano-toxicological studies. The results are strong evidence to develop potential antioxidant and anticancer silver nanoparticles for commercial application

Biography

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E-mail: vikilramesh131@gmail.com