Determination of effective parameters on growth rate and protein content of spirulina platensis under laboratory condition
Mohammad Gorgij Jaski, Kiuomars Rohani-Ghadikolaei, Mazeyar Yahyavei and Ali Reza Salarzadeh
Islamic Azad University, Iran
Iranian Fisheries Research Science Organization, Iran
: J Mar Biol Oceanogr
Abstract
In order to provide optimum growth and protein content, S. platensis cells were grown in modiï¬Âed Zarrouk (1966) media culture at different salinity (30, 32 and 35ppt), temperature (20, 23, 26 and 28ºC) and intensity (50, 75 and 90 µmol m-2s-1). The results of the present study clearly showed that the highest alga biomass and growth rate was obtained following culture under the 32 ppt salinity, 26°C temperature, and under a 13h light:11h dark photoperiod regime at a light intensity of 90 µmol m-2s- 1 provided by cool white fluorescent tubes (ï¬Âgure 1). Maximum alga biomass and growth rate of S. platensis in a 5 liter Erlenmeyer flask for twelve days reached to 8 gr L− 1 and 0.28 day- 1, respectively. However, the mean alga biomass of S. platensis in temperature regimes 20, 23, 26 and 28ºC (under salinity of 32 ppt, and 75 µmol m-2s-1 irradiance) were 4.72, 5.15, 7.69 and 7.03 gr L−1, respectively. The results clearly showed that S. platensis successfully cultivated under different physical condition and maximum protein content was produced in the 30 ppt salinity, 28°C temperature, and 75 µmol m-2s-1 irradiance. Based on the results from the present study, providing suitable media culture and physical condition can be considered as a promising method to S. platensis cultivation for achieving optimal biomass and protein production. sontderafshrimp@yahoo.com