Determination of effective parameters on growth rate and protein content of spirulina platensis under laboratory condition


Mohammad Gorgij Jaski, Kiuomars Rohani-Ghadikolaei, Mazeyar Yahyavei and Ali Reza Salarzadeh

Islamic Azad University, Iran

Iranian Fisheries Research Science Organization, Iran

: J Mar Biol Oceanogr

Abstract


In order to provide optimum growth and protein content, S. platensis cells were grown in modified Zarrouk (1966) media culture at different salinity (30, 32 and 35ppt), temperature (20, 23, 26 and 28ºC) and intensity (50, 75 and 90 µmol m-2s-1). The results of the present study clearly showed that the highest alga biomass and growth rate was obtained following culture under the 32 ppt salinity, 26°C temperature, and under a 13h light:11h dark photoperiod regime at a light intensity of 90 µmol m-2s- 1 provided by cool white fluorescent tubes (figure 1). Maximum alga biomass and growth rate of S. platensis in a 5 liter Erlenmeyer flask for twelve days reached to 8 gr L− 1 and 0.28 day- 1, respectively. However, the mean alga biomass of S. platensis in temperature regimes 20, 23, 26 and 28ºC (under salinity of 32 ppt, and 75 µmol m-2s-1 irradiance) were 4.72, 5.15, 7.69 and 7.03 gr L−1, respectively. The results clearly showed that S. platensis successfully cultivated under different physical condition and maximum protein content was produced in the 30 ppt salinity, 28°C temperature, and 75 µmol m-2s-1 irradiance. Based on the results from the present study, providing suitable media culture and physical condition can be considered as a promising method to S. platensis cultivation for achieving optimal biomass and protein production. sontderafshrimp@yahoo.com

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