Comparative study on the effects of two derivative of cucurbitacin B and I on antioxidant status in liver cancer cells line Huh-7

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Gilda Karimi, Masoud Mashhadi Akbar Boojar, Sarah Khavari-Nejad, Marziyeh Fathi Chesly

Kharazmi University, Iran

: J Pharm Sci Emerg Drugs

Abstract

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Cucurbitacin B (CuB) and Cucurbitacin I (CuI), has recently
emerged as a potent anticancer agent; however, all
aspects or its biological mechanism effects are still obscure
in which antioxidant indexes may be involved. Thus, our
objective was to determine the involvement or antioxidant
parameters and Paraoxonase activity in Huh-7 cell lines in
responses to two Cucurbitacin derivatives. In this study,
Huh-7 cells were treated with two derivatives of CuB
and CuI in order to measure the cell viability by the MTT
method. Malondialdehyde level (MDA), Catalase (CAT),
Superoxide dismutase (SOD), Glutathione peroxidase
(GPx) and Paraoxonase (PON1) activities were evaluated
by spectrophotometer and HPLC in cell extract after 24 h
treatment.

Biography

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Gilda Karimi is working as an assistant professor of molecular and cellular biochemistry in Department of Biological Sciences, Kharazmi University, Iran. She has completed her Ph.D. in Molecular Biochemistry, Faculty of sciences from University of Paris-Sud, France in 2010-2014. She has published many papers in reputed journals.

E-mail: g.karimi@khu.ac.ir