Antimicrobial effect of Chinese medicinal plant crude extracts against Rhizoctonia solani and Pythium aphanidermatum

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Muhammad Atiq, Nasir Ahmed Rajput, Nazir Javed, Yong-Hao Ye, Zhijian
Zhao, Rehana Naz Syed, Abdul Mubeen Lodhi, Babar Khan, Owais Iqbal and
Daolong Dou

University of Agriculture, Faisalabad, Pakistan
Nanjing Agricultural University, China
Yunnan Academy of Agricultural Sciences, China
Sindh Agriculture University, Pakistan

: J Plant Physiol Pathol

Abstract

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Medicinal plants have been found to natural anti-fungal and anti-oomycete compounds to defend plants against pathogenic organisms. Here we evaluated in vitro anti-fungal and anti-oomycete effect of the crude methanol extract from 103 Chinese medicinal plant species against Rhizoctonia solani and Pythium aphanidermatum. Twelve crude methanolic extracts showed strong anti-fungal and anti-oomycete activities against both pathogens using the paper disc diffusion method. The highest anti-fungal and anti-oomycete plant extract Glycyrrhiza uralensis that showed significant inhibitory effect against both pathogens and preferred for more assessment. Most effective solvent of methanolic extract from G. uralensis was separated through silica gel plates and each phase was evaluated for antifungal and anti-oomycete activity. The ethyl acetate phase of G. uralensis completely suppressed the radial growth of R. solani and P. aphanidermatum in vitro and in vivo. Taken together, our results indicate that ethyl acetate phase is an important antimicrobial constituent of G. uralensis, and that it’s anti-fungal and anti-oomycete effect is attributed to the control strategies of these pathogens.

Biography

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