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Hindi Research Article, J Pharm Drug Deliv Res Vol: 5 Issue: 3
Solvent Effect on Phenolic Compounds Extraction, Antioxidant Capacities and Α-Amylase and Α-Glucosidase Inhibition of Clematis flammula and Periploca angustifolia
| Khaled Athmouni1,2*, Taheni Belghith1,2, Abdelfattah El Feki2 and Habib Ayadi2 | |
| 1Faculty of Sciences of Sfax, University of Sfax, Department of life Sciences, de research Unit UR 11 ES 72/Biodiversity and Aquatic Ecosystems, Road Soukra Km 3,5, B.P. 1171, CP 3000 Sfax, Tunisia | |
| 2Faculty of Sciences of Sfax, Laboratory of Animal Ecophysiology, University of Sfax, P.O. Box 95, Sfax 3000, Tunisia | |
| Corresponding author : Khaled Athmouni University of Sfax, Faculty of Sciences of Sfax, Department of life Sciences, de research Unit UR 11 ES 72/Biodiversity and Aquatic Ecosystems, Road Soukra Km 3,5, B.P. 1171, CP 3000 Sfax, Tunisia Tel: +216 93242084 Fax: +216 74274437 E-mail: Khaled_athmouni@hotmail.fr |
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| Received: March 24, 2016 Accepted: September 12, 2016 Published: September 15, 2016 | |
| Citation: Athmouni K, Belghith T, Feki AE, Ayadi H (2016) Solvent Effect on Phenolic Compounds Extraction, Antioxidant Capacities and Α-Amylase andΑ-Glucosidase Inhibition of Clematis flammula and Periploca angustifolia. J Pharm Drug Deliv Res 5:3. doi:10.4172/2325-9604.1000152 |
Abstract
Solvent Effect on Phenolic Compounds Extraction, Antioxidant Capacities and Α-Amylase and Α-Glucosidase Inhibition of Clematis flammula and Periploca angustifolia
Abstract
Phytochemicals are extensively found at different levels in many medicinal plants. This work had three objectives: the first, to evaluate the total phenolic, flavonoids content, anthocyanins concentration and condensed tannins of two Tunisian medicinal plants, second, to assess their potential antioxidant activities using systems such as a, adiphenyl-b-picrylhydrazyl (DPPH• ), ABTS•+, O2 â??• , H2 O2 scavenging activity, Hydroxyl OH• and linoleic acid inhibition and third, to screen α-amylase and α-glucosidase inhibitors from common natural products found in traditional Tunisian medicines. The present study was performed that C. flammula (Ranunculaceae family)has the highest levels of total phenols followed by P. angustifolia (Asclepiadaceae family). On the other hand, the results show that the antiradical activity (DPPH) varies from 52 to 77% for P. angustifolia and 56 to 84% for C. flammula. The antioxidant activity measurement, expressed as Trolox equivalent antioxidant capacity (TEAC), ranged from 0.33 to 1.51 mM TE/ g DW. In general, all extracts exhibited good antioxidant activity. In the present investigation, the extracts most effective in inhibiting α-amylase (C. flammula and P. angustifolia) contain appreciable amounts of condensed tannins. In addition, the extent of inhibition of α-glucosidase was related to their anthocyanins content.
