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Hindi Research Article, J Immunol Tech Infect Dis Vol: 5 Issue: 2
Rv3881c from Mycobacterium tuberculosis Elicits PolyFunctional CD8+ T cells in PPD Positive Healthy Volunteers and Affords Significant Protection in the Guinea Pig Model
| Satchidanandam V1*, Kumar N1, Biswas S1, Jumani RS1, Jain C1, Rani R2, Aggarwal B2, Singh J2, Kotnur MR3, Challu V4, Chadha VK4, Kumar P4 and Sridharan A4 | |
| 1Department of Microbiology and Cell Biology, Indian Institute of Science, Sir C.V. Raman Avenue, Bangalore, Karnataka 560012, India | |
| 2Molecular Immunogenetics Group, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi 110067, India | |
| 3Department of Chest Medicine, MS Ramiah hospital, New BEL Road, Bangalore Karnataka 560054, India | |
| 4National Tuberculosis Institute, 8, Bellary Road, Bangalore, Karnataka 560003, India | |
| Corresponding author : Vijaya Satchidanandam Department of Microbiology and Cell Biology, Indian Institute of Science, Sir CV Raman Avenue, Bangalore, Karnataka 560012, India Tel: 91-80-22932685 Fax: 91-80-23602697 E-mail: vijaya@mcbl.iisc.ernet.in |
|
| Received: October 29, 2015 Accepted: March 04, 2016 Published: March 10, 2016 | |
| Citation: Satchidanandam V, Kumar N, Biswas S, Jumani RS, Jain C, et al. (2016) Rv3881c from Mycobacterium tuberculosis Elicits Poly-Functional CD8+ T cells in PPD-Positive Healthy Volunteers and Affords Significant Protection in the Guinea Pig Model. J Immunol Tech Infect Dis 5:2. doi:10.4172/2329-9541.1000138 |
Abstract
We show that Rv3881c, a secreted protein of M. tuberculosis (MTB), elicits T cell responses from healthy PPD-positive individuals and TB patients. Synthetic peptides spanning the complete length of Rv3881c induced secretion of significantly greater levels of TNF-α and IL-10 from peripheral blood mononuclear cells of TB patients compared to latently infect healthy volunteers. Polychromatic flow cytometry detected poly-functional CD4+ and CD8+ T cells secreting different combinations of IFN-γ, TNF-α, IL-2 and MIP-1β in response to Rv3881c. Levels of CD4+ T cells secreting either IFN-γ or TNF-α were significantly higher in TB patients while levels of CD8+ T cells secreting both IFN-γ and IL-2 were significantly greater in healthy PPD-positive volunteers.
