Immunohistochemical Markers of Angiogenesis and Apoptosis in Chorionic Villi during Early Pregnancy Miscarriage

Igor Zastavnyy¹, Antonina Yashchenko¹, Igor Shponka² and Iryna Tkach³

¹Danylo Halytsky Lviv National Medical University, Department of Histology, Cytology and Embryology, Lviv, Ukraine

²Dnipropetrovsk Medical Academy of Health Ministry of Ukraine, Department of Pathological Anatomy and Forensic Medicine, Dnipro, Ukraine

³Institute of Hereditary Pathology of National Academy of Medical Sciences of Ukraine, Lviv, Ukraine

*Corresponding Author : Igor Zastavnyy
Department of Histology, Cytology and Embryology, Danylo Halytsky Lviv National Medical University, Ukraine
Tel: +380322769373
E-mail: ihor.zastavnyy@gmail.coml

Received: December 12, 2017 Accepted: December 21, 2017 Published: December 28, 2017

Citation: Zastavnyy I, Yashchenko A, Shponka I, Tkach I (2017) Changes in the Expression of Immunohistochemical Markers of Angiogenesis and Apoptosis in Chorionic Villi during Early Pregnancy Miscarriage. Cell Biol (Henderson, NV) 6:2. doi: 10.4172/2324-9293.1000135

Objective: Aim of this study was to investigate changes in the expression of immunohistochemical markers of angiogenesis (VEGF) and apoptosis (Caspase 3) in chorionic villi of human embryos, obtained after sporadic, recurrent pregnancy miscarriage and compare them with such expression in normal pregnancy Chorionic villie.

Methods: Histological material included 63 recurrent, sporadic miscarriage and 32 artificial abortions chorionic villi samples. Immunohistochemical technique was applied to study expression levels of VEGF and Caspase 3.

Results: VEGF and Caspase 3 expression intensity and diversity in different Chorionic villie (CV) structural components was observed in different investigated groups.

 Conclusion: Our research has shown that an important role is played not only by a general decrease in VEGF expression but also a change in the allocation of this cytokine in the structural components of early pregnancy miscarriage CV. The processes of apoptosis were more active in the groups of early miscarriage; however, they did not block the synthesis of VEGF. These results may indicate the role of factors of angiogenesis and apoptosis in the development of early miscarriage of pregnancy.