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Hindi Research Article, Res J Zool Vol: 3 Issue: 5
Cytogenotoxic Effect Of PesticidesInduces Variability In Micronucleus And Nucleo- Cytoplasmic Abnormalities In Channa Punctatus In Vivo
The aquatic resource is the major part of our environment , its safety is directly related to our health. In this study, fresh water fish Channa punctatus was taken as the genotoxic test model to estimate water pollution using micronucleus (MN) assay. It has been used successfully as a mutagenic assay. The fish was exposed in vivo to three different concentrations(MC,MC/ 2&MC/5)of eight pesticides (Dimethoate,Dichlorovos,chlorpyriphos and Malath ion,Methyl parathion,Fenvalerate,Cypermethrin and Carbaryl) at different time periods (5,10,15,20,25days). Peripheral blood samples smears were stained with Giemsa,MN frequencies were qualitatively analyzed. Qualitative analysis of the output recommends the rate of concentration, period, nature and mode of action of different Agro- pesticides causes varieties of micronuclei and nucleo-cytoplasmic abnormalities in live fish Channa punctatus erythrocyte as sensitive indicator for evaluation and assessment of aquatic pollution.
Abstract
The aquatic resource is the major part of our environment , its safety is directly related to our health. In this study, fresh water fish Channa punctatus was taken as the genotoxic test model to estimate water pollution using micronucleus (MN) assay. It has been used successfully as a mutagenic assay. The fish was exposed in vivo to three different concentrations(MC,MC/ 2&MC/5)of eight pesticides (Dimethoate,Dichlorovos,chlorpyriphos and Malath ion,Methyl parathion,Fenvalerate,Cypermethrin and Carbaryl) at different time periods (5,10,15,20,25days). Peripheral blood samples smears were stained with Giemsa,MN frequencies were qualitatively analyzed. Qualitative analysis of the output recommends the rate of concentration, period, nature and mode of action of different Agro- pesticides causes varieties of micronuclei and nucleo-cytoplasmic abnormalities in live fish Channa punctatus erythrocyte as sensitive indicator for evaluation and assessment of aquatic pollution.
