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Hindi Research Article, J Nanomater Mol Nanotechnol Vol: 5 Issue: 6
An Investigation of In vitro Cytotoxicity of Silver Nanoparticles (Ag-NPs) on Cancerous Cell Lines
| Gowramma B, Dharaneeswara Reddy D and Muralidhara Rao D* | |
| Department of Biotechnology, Sri Krishnadevaraya University, Anantapuram, Andhra Pradesh, India | |
| Corresponding author : Muralidhara Rao D Department of Biotechnology, Sri Krishnadevaraya University, Anantapuram, Andhra Pradesh, India Tel: +919940699873 Fax: 08554 255244, 08554 255805 E-mail: muralidhararao@yahoo.com |
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| Received: May 03, 2016 Accepted: November 15, 2016 Published: November 20, 2016 | |
| Citation: Gowramma B, Dharaneeswara Reddy D, Muralidhara Rao D (2016) An Investigation of In vitro Cytotoxicity of Silver Nanoparticles (Ag-NPs) on Cancerous Cell Lines. J Nanomater Mol Nanotechnol 5:6. doi: 10.4172/2324-8777.1000204 |
Abstract
An Investigation of In vitro Cytotoxicity of Silver Nanoparticles (Ag-NPs) on Cancerous Cell Lines
Cytotoxicity and apoptotic effect of silver nanoparticles (Ag-NPs) was estimated in cancerous Hepatocellular carcinoma (HepG2), Human breast cancer (MCF-7), Human neuroblastoma (SK-N-SH) and Rat glioma (C6) cell lines, which were produced from Corynebacterium glutamicum. The studies of cytotoxicity were carried out under normoxia and hypoxia conditions. The cell lines of Ag-NPs cytotoxicity were demonstrated by MTT assay and half maximum inhibitory concentrations (IC50) were evaluated. Ag-NPs were exhibited good cytotoxic effects in C6 followed by HepG2, MCF-7 and SK-N-SH. Reactive Oxygen Species (ROS) production in all cell lines was examined. Analysis of apoptosis and necrosis was carried out by annexin-V/ PI staining method using flow cytometry. The present study was designed to recognize the anticancer property of Ag-NPs against HepG2, MCF-7, C6 and SK-N-SH and to the best of our knowledge this is the first report on cytotoxic activity of Ag-NPs synthesized from Corynebacterium glutamicum.
