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+44 28 3718 0055Journal of Genetic Disorders & Genetic ReportsISSN: 2327-5790
Bhambal A Author
Subjects of specialization
Affiliation
Human leukocyte antigens
Department of Public Health Dentistry, People
Biography
Mr. Bhambal A, belongs to the department of Public Health Dentistry, interest in the field of Human leukocyte antigens; Oral sub mucous fibrosis; Smoking; Tobacco, from People’s College of Dental Sciences, Peoples University, Bhopal, India
Publications
Research Article Subscription
Association of HLA Typing with Nicotine Dependence by Extraction of Salivary DNA among Indian Population
Author(s): Purohit A, Purohit BM, Mani A and Bhambal A
Objectives
Tobacco smoking is one of the major public health problems the world facing today. With more and more diseases being associated with alleles of the human leukocyte antigens (HLA) region than any other genetic region, objective of the study was to explore genetic association of smoking behavior with specific HLA Class II DQB1*0503 and HLA DRB1*0301 alleles.
Methods Genomic DNA was extracted from saliva of 39 patients divided among tobacco smokers and controls. This was followed by PCR with sequence specific primer of HLA-DQB1*0503 and HLA DRB1*0301 allele, visualized under 2% agarose gel.
Results
Significant difference was observed between tobacco smo... view more»
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