Abdullah M Alswieleh Author

Research Article Open Access

Quantitative Determination of Thiol Groups Modified Mesoporous Silica Nanoparticles by Ellman’s Reagent

Author(s):

Abdullah M Alswieleh 

Ellman's reagent 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB) is found to be a sensitive tool for the assay of thiol groups. DTNB reacts with aliphatic thiols to form fluorescent derivative “2-nitro-5-thiobenzoate (TNB−)”. In this work, Ellman's reagent was used to determine mercapto groups modified Mesoporous Silica Nanoparticles (MSNs). Mesoporous silica materials were prepared with a pore size of 5-6 nm, high surface area ca. 930 m2/g and a large pore volume of 1.3 cm3/g. MSNs were fabricated with 3-mercaptopropyl groups in both inner and outer surfaces. Such materials were reacted with DTNB in PBS solution to determine the concentration of 2-nitro5-thiobenzoate (TNB−) by colorimetric method. The concentration of thiol groups in the outer surface was... view more»

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