The Detection and Molecular Characterization of Canine Parvovirus Type-2 in Dogs with Gastroenteritis Symptoms in Ankara Province, Turkey

Parvoviruses can cause important and different viral diseases and infections in human and animals. Canine parvovirus infection is one of the infectious diseases with acute, fibrinous, necrotic or hemorrhagic enteritis which is common in dogs around the world. First canine parvovirus 2 reported in America in 1978. As a result of the analysis of CPV isolates with the monoclonal antibodies and enzymes, a strain (CPV2a) with its new antigenic properties appeared in America in 1979. In the following years, CPV2b was detected in 1984 in America and CPV2c in 2001 in Italy. In this study, identification, molecular characterization of the virus that is circulating in dogs at the same time in Turkey, determine the dominant type of virus and identify the location of virus in the phylogenetic tree and finally virus isolation was aimed. For this purpose, 100 samples were taken from dogs with gastroenteritis symptoms, among them, 52 of them were identified as PCR positive. Fifteen positive products were taken for next generation sequence and subjected to phylogenetic analysis. Five samples were observed as: CPV-2a, 9 samples as: CPV-2b and 1 sample as: CPV-2c. Seventeen CPVs were isolated in this study. In the phylogenetic viewpoint, TR-04, TR-06, TR-10, TR-11 and TR-15 constructs were identified as CPV-2a according to this analysis and were founded to be closely genetics related with FJ005257 (Italy), GU362934 (Italy), FJ005259 (Italy), KF385389 (Italy) and KF385390 (Italy). TR-01, TR-02, TR-03, TR-07, TR-08, TR-09, TR12, TR13 and TR-14 were included in the phylogenetic tree as CPV-2b and were founded to be closely genetics related with KF373569 (Italy), FJ222823 (Italy), FJ005264 (Italy), DQ025992 (France), FJ005260 (Germany) and KP682525 (Spain). TR-05 were located as CPV-2c and were found to be closely genetics related with DQ025942 (France), FJ005235 (Italy), DQ02956 (France) and FQ005246 (France) and in the order of GQ865518 (Greece), FJ005247 (Belgium), FJ005214 (Spain), FJ005237 FJ222821 (Italy) sequences. Fourteen of these parvovirus isolates can’t made cytopathic effect in CRFK cell line but 3 of them had cytopathic effect in CRFK cells line. Finally, with IF test, virus isolates were stabilized as: CPV type 2.