Subtractive Imaging Using Gaussian Fits and Image Moments in Confocal Microscopy

A subtractive imaging method is described that uses Gaussian fits and image moments in the CCD confocal microscopy configuration. Subtraction of the 0th order image moment of a digital pinhole filtered signal from the Gaussian fitted intensity signal reveals missing details of a high resolution test target. By choosing an appropriate weighting coefficient, the associated halo effect can be removed while retaining the fine details.