Nitella mirabilis from Assam: Morphology and Molecular Characterization using RAPD markers

Nitella mirabilis from Assam: Morphology and Molecular Characterization using RAPD markers

Exposure to ultraviolet-B radiation (UV-B) affects plants in various ways. This study was conducted to determine the comparative effects of exogenous nitric oxide (NO) and He-Ne Laser(5mWmm-2 )on alleviating effects of UV-B light (10.08 kJ•m–2•d–1) on ML7113 wheat development. Sodium nitro prusside (SNP) was selected as an exogenous nitric oxide (NO) donor，and the seed of wheat was presoaked with 0.1 mmol／L SNP for 24h．In this work, six treatment groups was set: control group (CK) ,ultraviolet-B treatment group (B), combined ultraviolet-B and He-Ne laser radiation treatment group (BL), laser treatment (L), combined ultraviolet-B and sodium nitro prusside treatment group (B+SNP) and sodium nitro prusside individual treatment group (SNP)．The results showed enhanced the UV-B leads to negative impact on growth and development of seedlings. Seedling growth for 7 days, the ultraviolet-B treatment of height, CAT activity, Fo, Fv and Fv/Fm decreased significantly compared with the control group while MDA content on the contrary. Exogenous nitric oxide and He-Ne Laser partially protected wheat seedlings from UV-B treatment damage. This finding results suggest that He-Ne laser has a stronger ability than exogenous nitric oxide to repair in UV-B radiation.