Modulation of Oxidative Stress Responsive Enzymes and Non-Enzymatic Components by Excess Nickel in C. lanatus Var.Fistulosus

Modulation of Oxidative Stress Responsive Enzymes and Non-Enzymatic Components by Excess Nickel in C. lanatus Var.Fistulosus

This study focuses on induction of oxidative stress and antioxidative defense mechanism on exposure to excess nickel (Ni) in C. lanatus L. var. fistulosus (C. lanatus). Plants grown in refined sand and treated with nickel (as nickel sulphate) at 0.0001 (control), 0.05, 0.1, 0.2, 0.4 and 0.5 mM. The primary site of Ni toxicity was shoots where young leaves developed interveinal chlorosis along with marked reduction in growth, after nine days of excess Ni supply (d 42). At excess Ni (>0.05 mM), biomass, concentrations of chlorophyll, carotenoides, iron, Hill reaction activity, and activity of catalase were decreased, whereas activity of peroxidase, ascorbate peroxidse, superoxide dismutase, proline and lipid peroxidation in leaves increased