Maryam Hamzeloo-Moghadam
Statement of the Problem: Processed Capparis spinosa fruits, have been
used in Iranian Traditional Medicine (ITM) for treatment of various
diseases; the fruits are believed to have hot and dry temperament. The
present study was designed in order to find the impact of processing
on some phytochemical constituents and biological activities of
Capparis spinosa. Methodology & Theoretical Orientation: The
fruits were collected from Parsabad Moghan, Ardebil, Iran (2016)
and processed with grape vinegar for 30 days. Total phenolics and
alkaloids contents of the raw and processed fruits were determined.
For quantitation of rutin and quercetin, R-HPLC with C18 column,
methanol: formic acid 1% as the mobile phase (gradient), ?max 257
nm was used. Cytotoxic activity of the fruits was determined by MTT
method. Antioxidant properties were evaluated by DPPH and FRAP
techniques. Fingerpriting of raw and processed fruits were prepared
using TLC with silica gel as the stationary phase and n-butanol: acetic
acid: H2O 40:10:10 as the mobile phase. Findings: Total phenolics
decreased in the processed fruits (7.5 times). Rutin concentration
did not change during processing but quercetin amount decreased.
Total alkaloids content was reduced as well. While, total phenolics
decreased, antioxidant activities of the processed fruits increased using
both DPPH and FRAP methods. No cytotoxic effect was observed for
the raw fruits on normal human fibroblast cells (AGO1522) up to
the concentration of 1000 ?g/mL; however, decrease in the viability
was observed for the processed fruits at this concentration. The
fingerprints of the fruits were different which admitted the change in
the fruit constituents due to processing. Conclusion & Significance: In
general, it seems that processing with vinegar reduced the unpleasant
taste of the plant due to alkaloids and increased the antioxidant effects;
therefore it would be more suitable for use in some diseases such as
diabetes and hepatitis.