Marker Assisted Selection for Bacterial Leaf Blight resistance in Segregating Populations of Karma Mahsuri

Marker Assisted Selection for Bacterial Leaf Blight resistance in Segregating Populations of Karma Mahsuri

Bacterial leaf blight (BLB) of rice is caused by Xanthomonas oryzae pv. oryzae (Xoo), this is a major disease of rice in several countries. The present investigation was undertaken with the objective to develop high yielding rice varieties possessing broad spectrum durable resistance by transferring bacterial leaf blight (BLB) resistant genes viz., xa5 xa13 and Xa21 from a donor IRBB59 and a popular high yielding rice variety i.e., Karma Mahsuri, susceptible to BLB and were selected as parents. The presence of target genes by using gene linked primers viz., xa5R, xa5S, xa13 promoter and Xa21F/R was done. Segregating population was advanced to BC2 F3 generation and foreground selection was done using gene linked markers. Genetic analysis in BC2 F3 populations confirmed the presence of genes (xa5, xa13 & Xa21) governing BLB resistance in 22 lines. p>