In-Vitro Determination of the Cytotoxicity and Antiproliferative Activity of the Semi-Purified Flavonoids from the Bauhinia Malabarica (Alimbangbang) Leaves Extract against Selected Cancer Cell Lines

The study aims to determine the cytotoxicity and anti-proliferative property of the semi-purified flavonoids of Bauhinia malabarica. In order to achieve this, the plant material undergone various experiment and tests. 80% Ethanol was used in the extraction process through maceration. The crude extract gave a brownish yellow liquid with characteristic odor. The semi-purification process was carried out giving a brownish black dried sample. Shinodas, Sodium hydroxide, Ethyl acetate, and lead acetate was used for confirmatory testing to determine the presence of flavonoids .Results showed a 0.24% of percent yield of flavonoids obtained from the 500-gram plant sample. FT-IR results showed absorbance with functional groups of alkane, acid, and aromatic bonding. HPLC results showed the active constituent percent in the extract is quercetin with 0.99%. Brine Shrimp Assay showed significant results against nautili eggs. Unfortunately, the MTT Assay in both cancer cell lines revealed inactive against MCF-7 and A549.