In vitro Production of L-DOPA In Tissue Cultures of Mucuna pruriens L.

Callus cultures and regenerated plants of Mucuna pruriens L. were selected for the enhancement of L-DOPA. Treatment of cultures was carried out with an amino acid tyrosine as precursor and with NaCl. High percentage of L-DOPA was observed in regenerated plants grown under in vitro conditions on MS medium, supplemented with 50mM NaCl as compared to regenerated plants on basal MS medium and also as compared to the callus raised on MS medium supplemented with different concentration of PGRs with or without tyrosine and NaCl or in combination of both. The study suggests that in vitro regenerated plant of Mucuna pruriens is a high accumulator of L-DOPA under salt stress.