Improvement in Regeneration Potentiality by Using Magnesium Nitrate Primed Seeds of Rice In vitro

Improvement in Regeneration Potentiality by Using Magnesium Nitrate Primed Seeds of Rice In vitro

Plants raised from magnesium nitrate [Mg(NO3)2] primed seeds showed stress ameliorating characters. On the basis of this, during the study of somatic embryogenesis a comparative characterization of the calli were made by using the dehusked, sterilized whole rice (var-Swarna/ MTU-7029) seeds, obtained from Mg(NO3)2 primed and non-primed one. For callus induction 30gL-1 sucrose + 1gL-1 casein hydrolysate and 30gL-1 maltose + 1gL-1 casein hydrolysate were added in MS medium separately which was supplemented with 2 mgL-1 2,4-D. In the regeneration medium different concentration of BAP, kinetin and NAA were used. For the regeneration of roots 1mgL-1 IBA was used. Different concentrations (2-8 mM) of the Mg(NO3)2 used for priming before using the seeds on culture medium, showed promising results regarding the high callus induction frequency with faster greening ability and stout shoots in respect to non primed one. 4 mM of Mg(NO3)2 was found best among all nitrate treatment. The fresh and dry weights and proline content were found maximum in the calli obtained from the 4 mM Mg(NO3)2 primed seeds. The Mg(NO3)2 primed sets have also more number of roots reflected more rooting ability in respect to non- primed one.