Implications of Three Dimensional Cell Culture in Drug Discovery

According to its numerous advantages in delivering more physiologically appropriate information and more predictive data for in vivo studies, three-dimensional (3D) cell culture techniques have attracted growing interest in drug development and tissue engineering. In this study, the differences between two-dimensional (2D) monolayer culture and three-dimensional (3D) cell culture methods, with a particular emphasis on cell growth conditions, cell proliferation, population, and gene and protein expression patterns.