Guide for Designing Species Specific Primer

To identify, characterize and differentiate the pathogen strains, a PCR-based technique is developed. In this method, speciesspecific primers need to be designed with which we can know whether a particular pathogen strain is present or not among a group. To design such types of primers, we need sequences of the related species. All these sequences are aligned and a unique SNP that differentiates a particular species from the rest is used for designing a specific primer. A protocol has been developed for this; by following this researchers can easily identify their desired strain and can use it for breeding purposes.