Epigenesis Through In-vitro Regeneration in Soybean Amenable to Genetic Transformation

Epigenesis Through In-vitro Regeneration in Soybean Amenable to Genetic Transformation

An efficient and reproducible plant regeneration procedure is essential for introducing foreign gene (s) of interest in important crops through genetic transformation. However, some crops, such as soybean [Glycine max (L.) Merrill.] are difficult to manipulate in vitro, often depending on their genotype under study, and the reproduction of the established protocols is not always possible. The purpose of this paper is the optimization of a regeneration protocol for soybean shoots of the Indian variety JS-335 to enable its reproduction. Four explants viz, Cotyledonary node, embryo, half seed and hypocotyl were tested using various combinations and concentrations of growth hormones. The effect of several concentrations of 2,4-D and BAP was effective in initiating Callogenesis in three types of explants though concentration varied widely. The highest callogenesis of 82.93% were recorded on 14th day using half seed explant. However, the highest shoot induction of 93.79% with multiple numbers of shoots was recorded using cotyledonary node explant inoculated on the medium fortified with 5.0 mg.L-1 BAP. .