Effect of a Potent Cytokinin Thidiazuron (TDH) on the Regeneration Potential of Leaf and Petiole Explants of Cauliflower (Brassica oleracea L var. botrytis)

Effect of a Potent Cytokinin Thidiazuron (TDH) on the Regeneration Potential of Leaf and Petiole Explants of Cauliflower (Brassica oleracea L var. botrytis)

Plant regeneration studies were carried out using leaf and petiole explants on forty different combinations and concentrations of TDZ i.e. TDZ, TDZ+IAA, TDZ+NAA and TDZ+Adenine. Among the forty combinations tried, the high percent shoot regeneration was observed in petiole explants (76%) on MS medium supplemented with 0.22mg/l TDZ whereas in case of leaf explants only 44% shoot regeneration was achieved on MS medium supplemented with 0.77mg/l TDZ+ 0.087 mg/l IAA. For root regeneration the in vitro regenerated shoots were cultured on MS medium containing different concentration of IAA and NAA. Hundred percent root regeneration was observed on the MS medium supplemented with 0.05mg/l and 0.20mg/l NAA respectively. The regenerated plantlets were transferred to the pots containing cocopeat and acclimatized. The regenerated plantlets were morphologically similar. An efficient and reproducible plant regeneration protocol has been standardized in cauliflower cv. Pusa Snowball K-1 and is being used for Agrobacterium- mediated cry1Aa gene transfer studies.