Development of A SYBER Green Real-Time

Swine viral diseases are of great concern as they hamper the economy of the swine industry. One of such major disease problem is porcine parvovirus (PPV) infection cause reproductive failures in swine. Porcine parvovirus is an autonomously replicating parvovirus, belongs to genus parvovirus from the parvoviridae family. Qualitative real time PCR is a method to rapidly and precisely detect and quantify gene of interest. In the present study we have developed an assay based on real time PCR for the screening of porcine parvovirus in tissue DNA samples