Bio-Removal of Vat Dye from Textile Effluent by Candida tropicalis and Candida apis Isolated from Soil

 Bio-Removal of Vat Dye from Textile Effluent by Candida tropicalis and Candida apis Isolated from Soil

The bio-removal of textile effluent (Vat dye) by yeasts isolated from soil was studied. A total of 5 yeasts were isolated and identified from soil (using morphological and biochemical characterization) to remove vat dye from textile effluent. These isolates were screened by inoculating them into 500 ml conical flask each containing sterile mineral salt media and 20 mg of vat dye. Of these isolates, Candida tropicalis and Candida apis stood out as potential dye decolorizes after incubation. Candida apis and Candida tropicalis removed 90.6% and 84.1% of color from the textile effluent after incubating for 25 days at 37ºC. The textile effluent treated had initial high concentration of Biochemical Oxygen Demand (BOD), Chemical Oxygen Demand (COD), and Nitrate, above the standard acceptable limit which is an indication of high contamination. All the isolates used performed efficiently in reducing the concentration of these parameters in the textile effluent at neutral pH of 7.0. The Yeast isolates (Candida apis and Candida tropicalis) exhibited good potentials in the treatment of textile effluent. Candiada apis reduced the BOD from 1,425 mg/l to 272 mg/l COD from 3,550 mg/l to 679 mg/l while the Nitrate was reduced from 255 mg/l to 65 mg/l and Candida tropicalis reduced the BOD from 1,425 mg/l to 312 mg/L, the COD was reduced from 3,550 mg/l to 780 mg/l and the Nitrate was reduced from 255 mg/l to 78 mg/L. These microbial isolates can be recommended as good candidates for treatment of waste water, especially textile waste water..