Analysis of Virulence Associated and Antibiotic Resistance Genes of Microbes in Subclinical Mastitis Affected Cattle Milk by Pyrosequencing Approach

Analysis of Virulence Associated and Antibiotic Resistance Genes of Microbes in Subclinical Mastitis Affected Cattle Milk by Pyrosequencing Approach

Metagenomic analysis of milk samples collected from Gir, Kankrej (Bos indicus) and crossbred (Bos taurus X Bos indicus) cattle harbouring subclinical mastitis was carried out by pyrosequencing technology to elucidate virulence associated genes and sequences from the microbial community. Metabolic profile by a web-based tool MG-RAST revealed that members of Virulence, disease and defense associated genes were found to be 3.53,2.88 and 5.26 per cent (the percent values were calculated by finding its proportional hits among total hits obtained) in Gir, Kankrej and crossbred cattle, respectively.