An Investigation of In vitro Cytotoxicity of Silver Nanoparticles (Ag-NPs) on Cancerous Cell Lines

An Investigation of In vitro Cytotoxicity of Silver Nanoparticles (Ag-NPs) on Cancerous Cell Lines

Cytotoxicity and apoptotic effect of silver nanoparticles (Ag-NPs) was estimated in cancerous Hepatocellular carcinoma (HepG2), Human breast cancer (MCF-7), Human neuroblastoma (SK-N-SH) and Rat glioma (C6) cell lines, which were produced from Corynebacterium glutamicum. The studies of cytotoxicity were carried out under normoxia and hypoxia conditions. The cell lines of Ag-NPs cytotoxicity were demonstrated by MTT assay and half maximum inhibitory concentrations (IC50) were evaluated. Ag-NPs were exhibited good cytotoxic effects in C6 followed by HepG2, MCF-7 and SK-N-SH. Reactive Oxygen Species (ROS) production in all cell lines was examined. Analysis of apoptosis and necrosis was carried out by annexin-V/ PI staining method using flow cytometry. The present study was designed to recognize the anticancer property of Ag-NPs against HepG2, MCF-7, C6 and SK-N-SH and to the best of our knowledge this is the first report on cytotoxic activity of Ag-NPs synthesized from Corynebacterium glutamicum.