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Hindi Research Article, J Immunol Tech Infect Dis Vol: 5 Issue: 1
Standardization of a Multiplex Magnetic Bead-based for Simultaneous Detection of IgG to Plasmodium Antigens
| Perraut R1*, Varela M-L1, Mbengue B2, Guillotte M3, Mercereau-Puijalon O3 and Vigan-Womas I3 | |
| 1Institute Pasteur of Dakar, Immunology Unit, Dakar, Senegal | |
| 2Institute Pasteur de Dakar, Immunology Unit, Dakar, Senegal | |
| 3Institute Pasteur, Molecular Immunology Unit of Parasite, 25-28 Rue du Dr Roux, 75015 Paris, France | |
| Corresponding author : Ronald Perraut Institute Pasteur de Dakar, Immunology Unit, Dakar, Senegal E-mail: perraut@pasteur.sn |
|
| Received: October 01, 2015 Accepted: December 08, 2015 Published: December 14, 2015 | |
| Citation: Perraut R, Varela M-L, Mbengue B, Guillotte M, Mercereau-Puijalon O, et al. (2016) Standardization of a Multiplex Magnetic Bead-based for Simultaneous Detection of IgG to Plasmodium Antigens. J Immunol Tech Infect Dis 5:1. doi:10.4172/2329-9541.1000134 |
Abstract
Standardization of a Multiplex Magnetic Bead-based for Simultaneous Detection of IgG to Plasmodium Antigens
Multiplex assays are currently used to facilitate the evaluation of antibody (Ab) responses to multiple Plasmodium falciparum antigens from large field-based epidemiological studies. The present study aimed at (i) optimizing parameters of a novel cost-effective, compact and reliable magnetic bead-based multiplex immunoassay (MBA) carried out with the MAGPIX®-Luminex system and (ii) comparing the results with those obtained using standard ELISA technology.
