The Journal of Biocatalysis & Biotransformation (JBCBT) promotes rigorous research that makes a significant contribution in advancing knowledge for the catalytic activities of an enzyme. JBCBT includes all major themes pertaining to the series of chemical reactions that occur in living organisms to produce a compound.
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Parametric Optimization of Extracellular Chitin Deacetylase Production by Scopulariopsis brevicaulis
The culture medium, fermentation conditions and effects of different inorganic salts on producing extracellular chitin deacetylase by Scopulariopsis brevicaulis were optimized.The highest enzymatic activity of deacetylation of chitin was 36 units/mL for optimum culture medium which included 2% (w/v) 3,6-O-carboxymethylchitin, 1% sucrose, 0.2% NaNO3, 0.1% K2HPO4, 0.05% KCl, 0.4% peptone, 0.05% MgSO4, 0.05% MnSO4, 0.03% CoCl2 and optimum cultural conditions included temperature, pH, agitation speed, fermenting time and volume and were 29°C, 7.0, 200 rmp min-1, 96 hours and 80 mL in 300 mL Erlenmeyer flask, respectively
From a commercial enzyme extract juice with purpose to stabilize it in the industry, has been able to extract an extract rich in beta-galactosidase activity. It has been designed to extract and purification protocol based on polyethyleneimine SUPPORTS agarose activated with next purification yields 80%.
Xylanase Production from Aspergillus japonicus var aculeatus: Production using Agroindustrial Residues and Biobleaching Effect on Pulp
This study describes a xylanase produced by A. japonicus var aculeatus, and evaluated the suitability of this protein for cellulose pulp biobleaching process. The best production of xylanase was achieved with wheat bran (AE=11 U/mg of protein).
Immobilization of Haloalkane dehalogenase LinB from Sphingobium japonicum UT26 for Biotechnological Applications
Haloalkane dehalogenases are enzymes capable of converting a broad range of aliphatic halogenated compounds to corresponding alcohols. These dehalogenase-based biotransformations are attractive for various biological processes, e.g. biocatalysis, bioremediation and detoxification, which often require protein immobilization.
Influence of Macromolecular Crowding on the Oxidation of ABTS by Hydrogen Peroxide Catalyzed by HRP
The interior of the living cell is highly concentrated and structured with molecules having different shapes and sizes. However, almost all experimental biochemical data have been obtained working in dilute solutions that do not reflect in vivo conditions.